Singson Lab

Reproductive biology, fertilization, and cell-cell interactions in C. elegans

Waksman Institute | Rutgers University

Publications

  1. J. M. Parry, A. J. Lefkovith, J. S. Hang, J. Ohm, R. Klancer, M. H. Zegarek, R. Maruyama, N. V. Velarde, F. Piano, B. D. Grant and A. Singson (2009). EGG-4 and EGG-5 link events of the oocyte-to-embryo transition with meiotic progression in C. elegans. Current Biology. 19: 1752-1757. Pubmed abstract
    This manuscript and Cheng et al. (2009) are featured in a Dispatch: C. S. Heighington and E. T. Kipreos (2009) Embryogenesis: Degenerate phosphatases control the oocyte-to-embryo transition. Current Biology. 19: R939-R942. Pubmed abstract
  2. K. Chih-Chien Cheng, R. Klancer, A. Singson and G. Seydoux. (2009) Regulation of MBK-2/DYRK by CDK-1 and the pseudophosphatases EGG-4 and EGG-5 during the oocyte-to embryo transition. Cell. 139: 560-572. Pubmed abstract
    This manuscript and Parry et al. (2009) are featured in a Leading Edge Preview: N. K. Tonks (2009) Pseudophosphatases: Grab and hold on. Cell. 139: 464-465. Pubmed abstract
  3. P. Kadandale and A. Singson. (2009) The life of EGG-2. Visions: the art of science. Molecular Reproduction and Development. 76: 429. Publisher link
  4. P. Kadandale, I. Chatterjee, A. Singson. (2009) Germline transformation of Caenorhabditis elegans by injection. Methods in Moleclar Biology. 518: 123-133. Pubmed abstract
  5. A. Singson, J.S. Hang and J.M. Parry. (2008) Genes required for the common miracle of fertilization in Caenorhabditis elegans. International Journal of Developmental Biology. 52: 647-656. Pubmed abstract
  6. J.S. Hang, B.D. Grant and A. Singson. (2008) Meiotic Maturation: Receptor Trafficking Is the Key. Current Biology 18: R416-R418. Pubmed abstract
  7. R. Maruyama, N. V. Velarde, R. Klancer, S. Gordon, P. Kadandale, J. M. Parry, J. S. Hang, J. Rubin, A. Stewart-Michaelis, P. Schweinsberg, B. D. Grant, F. Piano, A. Sugimoto and A. Singson. (2007) EGG-3 regulates cell-surface and cortex rearrangements during egg activation in Caenorhabditis elegans. Current Biology. 17: 1555-1560. Pubmed abstract
    This manuscript was featured in a Dispatch: J. A. Govindan and D. Greenstein (2007) Embryogenesis: Anchors away! Current Biology. 17: R890-R892. Pubmed abstract
  8. R. Maruyama and A. Singson. (2006) Taking care of Dad's DNA. Genome Biology 7: 244. Pubmed abstract
  9. I. Chatterjee, P. Kadandale and A. Singson. (2006) Meiotic diapause: how a sperm signal sets you free. Current Biology 16: R496-R499. Pubmed abstract
  10. A. Singson. (2006) Sperm activation: time and tide wait for no sperm. Current Biology 16: R160-R162. Pubmed abstract
  11. B. Geldziler, I. Chatterjee, P. Kadandale, E. Putiri, R. Patel and A. Singson. (2006) A comparative study of sperm morphology, cytology and activation in Caenorhabditis elegans, Caenrohabditis remanei and Caenorhabditis briggsae. Development, Genes and Evolution 216: 198-208. Pubmed abstract
  12. E. J. Gleason, W. C. Lindsey, T. L. Kroft, A. W. Singson and S. W. L’Hernault. (2006) spe-10 Encodes a DHHC-CRD zinc finger membrane protein required for ER/golgi membrane morphogenesis during Caenorhabditis elegans spermatogenesis. Genetics 172: 145-158. Pubmed abstract
  13. P. Kadandale, A. Stewart-Michaelis, S. Gordon, J. Rubin, R. Klancer, P. Schweinsberg, B. D. Grant and A. Singson. (2005) The egg surface LDL-receptor-repeat containing proteins EGG-1 and EGG-2 are required for fertilization in Caenorhabditis elegans. Current Biology 15: 2222-2229. Pubmed abstract
  14. P. Kadandale, B. Geldziler, M. Hoffman, and A. Singson. (2005) Use of SNPs to determine the breakpoints of complex deficiencies, facilitating gene mapping in Caenorhabditis elegans. BMC Genetics 2005, 6:28. Pubmed abstract | Publisher abstract | Article PDF
  15. B. Geldziler, I. Chatterjee, and A. Singson. (2005) The genetic and molecular analysis of spe-19, a gene required for sperm activation in Caenorhabditis elegans. Developmental Biology 283: 424-436 . Pubmed abstract
  16. I. Chatterjee, A. Richmond, E. Putiri, D. C. Shakes, and A. Singson. (2005) The Caenorhabditis elegans spe-38 gene encodes a novel four pass integral membrane protein required for sperm function at fertilization. Development, 132: 2795-2808. Pubmed abstract | Publisher abstract
  17. E. Putiri, S. Zannoni, P. Kadandale, and A. Singson. (2004) Functional domains and temperature sensitive mutations in SPE-9, an EGF repeat containing protein required for fertility in Caenorhabditis elegans. Developmental Biology. 272: 448-459. Pubmed abstract | Publisher fulltext
  18. P. Kadandale and A. Singson. (2004) Oocyte production and sperm utilization patterns in semi-fertile strains of Caenorhabditis elegans. BMC Developmental Biology. 2004 Apr 15; 4(1):3. Pubmed abstract | Publisher abstract | Article PDF
  19. B. Geldziler, P. Kadandale and A. Singson. (2004) Molecular genetic approaches to studying fertilization in model systems. Reproduction. 127: 409-416. Pubmed abstract | Publisher abstract | Article PDF
  20. S. Zannoni, S. W. L'Hernault and A. W. Singson (2003) Dynamic localization of SPE-9 in sperm: a protein required for sperm-oocyte interactions in Caenorhabditis elegans. BMC Developmental Biology. 2003 Dec 3; 3(1):10. Pubmed abstract | Publisher abstract | Article PDF
  21. J. Bandyopadhyay, J. Lee, J. Lee, J. I. Lee, J. R. Yu, C. Jee, J. H. Cho, S. Jung, M. H. Lee, S. Zannoni, A. Singson, H. S. Koo and J. Ahnn. (2002) Calcineurin, a component of G-protein coupled phosphorylation pathways, is involved in movement, fertility, egg laying and growth in C. elegans. Molecular Biology of the Cell. 13: 3281-3293. Pubmed abstract | Publisher abstract
  22. B. Park, D. Lee, S. Jung, J. Yu, K. Choi, J. Y. Kwon, J. Lee, J. Lee, A. Singson, W. K. Song, C. S. Park, D. H. Kim, J. Bandyopadhyay and J. Ahnn. (2001) Calreticulin, a calcium-binding molecular chaperone is required for stress response and sperm fertility in C. elegans. Molecular Biology of the Cell. 12: 2835-2845. Pubmed abstract | Publisher abstract
  23. A. Singson, P. Kadandale and S. Zannoni. (2001) Molecules that Function in the Steps of Fertilization. Cytokine & Growth Factor Reviews. 12: 299-304. Pubmed abstract | Article PDF
  24. R. E. Navarro, E. Y. Shim, Y. Kohara, A. Singson and T.K. Blackwell. (2001) cgh-1, a Conserved Predicted RNA Helicase Required for Gametogenesis and Inhibition of Germline Apoptosis in' C. elegans. Development. 128: 3221-3232. Pubmed abstract | Publisher abstract
  25. A. Singson. (2001) Every Sperm is Sacred: Fertilization in C. elegans. Developmental Biology. 230: 101-109. Pubmed abstract | Article PDF
  26. S. W. L'Hernault and A. Singson. (2000) Developmental Genetics of Spermatogenesis in the Nematode Caenorhabditis elegans. In: The Testes: From Stem Cell to Sperm Function, Serono Symposium USA, 109-119.
  27. A. Singson, K. L. Hill and S. W. L'Hernault. (1999) Sperm Competition in the Absence of Fertilization in Caenorhabditis elegans. Genetics. 152: 201-208. Pubmed abstract | Publisher abstract | Article PDF
  28. A. Singson, K. B. Mercer and S. W. L'Hernault. (1998) The C. elegans spe-9 Gene Encodes a Sperm Transmembrane Protein that Contains EGF-Like Repeats and is Required for Fertilization. Cell. 93: 71-79. Pubmed abstract | Publisher abstract | Article PDF